H5N1 virus causes significant perturbations in host proteome very early in influenza virus-infected primary human monocyte-derived macrophages.
Cheung, C.Y., E.Y. Chan, A. Krasnoselsky, D. Purdy, A.T. Navare, J.T. Bryan, C.K.L. Leung, K.P.Y. Hui, J.S.M. Peiris, and M.G. Katze
H5N1 influenza viruses cause disease in humans with unusually high pathogenicity. We used mass spectrometry-based quantitative proteomic profiling of the temporal response of primary human monocyte-derived macrophages infected with highly pathogenic H5N1 and seasonal H1N1 influenza viruses to demonstrate significant perturbation of the host proteome upon viral infection, as early as 1 hour after infection. This early host response distinguished H5N1 from H1N1 infection, the latter inducing less of a response. The most pronounced effect was observed on the translational machinery suggesting that H5N1 might gain advantage in replication by utilizing the cell protein synthesis machinery early in the infection.
1. Differentially expressed peptides by time point.
2. Detected peptides with associated annotation. Column headers refer to the infection condition (mock, M; H1N1, H5N1), replicate number and time point when the individual sample was collected.