WHAT'S NEW @ KATZE LAB
PRESS
Rhesus macaque genome may hold clues for human health and evolution
1918 flu ravaged body's defenses
Research on monkeys finds resurrected 1918 flu killed by turning the body on itself
Study uncovers lethal secret of 1918 influenza virus
Publication highlights
Two recent publications report on the use of mass spectrometry based approaches to quantify cellular protein abundance changes in response to virus infection. These studies were conducted in collaboration with Dr. Richard Smith's group at Pacific Northwest National Laboratory and represent significant technical advances in the application of proteomic technologies to HIV-1 and HCV research.
Chan et al., Journal of Virology 81:7571-7583, 2007
Eric Chan et al. report on the first large-scale quantitative analysis of protein abundance changes in a CD4+ T cell line infected with HIV-1. This study quantified over 3,200 proteins and revealed changes in the abundance of proteins associated with nuclear transport, ubiquitination, and cell-cycle progression. In addition, changes in the abundance of cellular proteins known to interact with HIV-1 proteins were identified.
Diamond et al., Hepatology 46:649-657, 2007
Deborah Diamond et al. describe the use of an ultra-sensitive proteomics platform for performing quantitative protein profiling on microgram amounts of HCV-infected human liver tissue obtained from patients at different stages of fibrosis. This study quantified over 1,600 proteins and revealed protein abundance changes that are associated with fibrosis stage. Functional analysis of these proteins suggests that impairment of key mitochondrial processes, including fatty acid oxidation and response to oxidative stress, occurs during advanced fibrosis.